LOCUS       sequence-108107-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes N. meningiditis dCas9 with c-terminal fusion of human p300 
            HAT core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA3.3-Nm-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..36)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggttcaagaa gcgtagtccg gaacgtcgta cgggtagtcc tggctctgcg tgtgcagctc
       61 caccagcatg cacatggtgg accactgggc tcttcggagt gaagagaact ccaggtgctt
      121 gtcccttgcc agcgtgagaa acgcatcccg accatccatc agatcgcagg ggatgagagg
      181 atcaggatca acaatgggag gcagggagtt ggcagcaggg ccagcaatga ggcggatcac
      241 aaagaagacc tctttatgct tctccatggt ggcatatagt ttctgtgaga ggtcgttaga
      301 tacattgggc atcccgggtt tcttcttgtt gcccctactc aggctgctct tatttttgct
      361 ggttttctta ttattcttct ttttagcatt tttgctgtct cccttggtca catctgtgct
      421 ttcattgctg gtgttttcct ctcgttttct ctcttcttcc tcctgttcca gttccttaat
      481 gctttcttcc agaacattgg gccagaaatc accctcgaaa taaggcaatt cctttgcact
      541 tgttaatcta tcttcagtag cttgtttaaa aatatccttg tagtcatgga caatacgctc
      601 tgatacagcc ttgtcaagca tttttttgta ccattcctgc agtcgcttgg gctt
//
