LOCUS       sequence-10811-e        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PSKH1
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1121..1129
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgggctgtg ggacaagcaa ggtccttccc gagccaccca aggatgtcca gctggatctg
       61 gtcaagaagg tggagccctt cagtggcact aagagtgacg tgtacaagca cttcatcaca
      121 gaggtggaca gtgttggccc tgtcaaagcc gggttcccag cagcaagtca gtatgcacac
      181 ccctgccccg gtcccccgac tgctggccac acggagcctc cctcagaacc accacgcagg
      241 gccagggtag ctaagtacag ggccaagttt gacccacgtg ttacagctaa gtatgacatc
      301 aaggccctaa ttggccgagg cagcttcagc cgagtggtac gtgtagagca ccgggcaacc
      361 cggcagccgt atgccatcaa gatgattgag accaagtacc gggaggggcg ggaggtgtgt
      421 gagtcggagc tgcgtgtgct gcgtcgggtg cgtcatgcca acatcatcca gctggtggag
      481 gtgttcgaga cacaggagcg ggtgtacatg gtgatggagc tggccactgg tggagagctc
      541 tttgaccgca tcattgccaa gggctccttc accgagcgtg acgccacgcg ggtgctgcag
      601 atggtgctgg atggcgtccg gtatctgcat gcactgggca tcacacaccg agacctcaaa
      661 cctgagaatc tgctctacta ccatccgggc actgactcca agatcatcat caccgacttc
      721 ggcctggcca gtgctcgcaa gaagggtgat gactgcttga tgaagaccac ctgtggcacg
      781 cctgagtaca ttgccccaga agtcctggtc cgcaagccat acaccaactc agtggacatg
      841 tgggcgctgg gcgtcattgc ctacatccta ctcagtggca ccatgccgtt tgaggatgac
      901 aaccgtaccc ggctgtaccg gcagatcctc aggggcaagt acagttactc tggggagccc
      961 tggcctagtg tgtccaacct ggccaaggac ttcattgacc gcctgctgac agtggaccct
     1021 ggagcccgta tgactgcact gcaggccctg aggcacccgt gggtggtgag catggctgcc
     1081 tcttcatcca tgaagaacct gcaccgctcc atatcccaga acctccttaa acgtgcctcc
     1141 tcgcgctgcc agagcaccaa atctgcccag tccacgcgtt ccagccgctc cacacgctcc
     1201 aataagtcac gccgtgtgcg ggaacgggag ctgcgggagc tcaacctgcg ctaccagcag
     1261 caatacaatg gcttg
//
