LOCUS       sequence-108110-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter encoding a mutated fragment of the
            SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
       61 ggccaagaag ggcggaaagt ccaaattgct cgagtgatga aagctgcgca ctagtgaggg
      121 agctcatagg ccggcataga cgcgttgaaa aactgtttaa ctcatgtcat aatgtgtctc
      181 ctcagatata gttaactagc ttgatagttt tgagtatata atgtaaataa tacataataa
      241 gtttgtaacc atttctgtta cttcataccc gtgtaattgg acatagcaga tgattatcat
      301 aagaatttgt tttaatggtt tttttccctc ctggaattac ctgattaaac ctgtcatgaa
      361 ttataaaagg actttttctt attctcctag agcttatcaa attatcacat aagagataat
      421 tactggagga gaaagtaaaa atggaagctt ttgataatta gctctgttct ttaaaagtat
      481 acttttaaac cgaaagttca catatagttc atcttaataa catatttatt catccttaat
      541 tgtattcaga ttttttttta agtctctaga tcaataatgt tatatttatt ggtttggtaa
      601 catgttgcag ctaagctaat gaccttaagt ggcaattgtt taacccagga ctactgattt
      661 ttttatattc aagtcagttt cattgtttta cttaactcgc catataacca aaaataacga
      721 ataatctaaa ggaaaggtta ttaggacagt aataaaaaat tatagtgtac ctcaacacta
      781 gaataagttg gattactata ttatagttta tttgaaaaat caaggagtaa ctgtatcttt
      841 accattaatt atgaaatgta gtctcatgtg cttatttaca gg
//
