LOCUS       sequence-108112-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LIC E coli expression plasmid to produce TEV cleavable His tagged 
            iLOV fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     B Smith Lab iLOF fusion plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNIC28-Bsa4-iLOVf
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..170
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             519..536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             561..581
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat acataatgaa tcacaaagtg atgatcggta
      181 ccattgagaa gaatttcgta atcactgatc cacgtctgcc ggacaacccg atcatcttcg
      241 cttccgacgg cttcctggag ctgaccgagt attcccgtga agagatcctg ggccgtaacg
      301 cccgtttcct gcagggcccg gagactgatc aggcgacggt tcagaaaatt cgcgatgcta
      361 tccgtgacca gcgtgaaact acggtgcaac tgattaacta cacgaaaagc ggtaagaaat
      421 tctggaacct gctgcatctg caaccagtcc gcgatcagaa aggcgagctg cagtatttca
      481 tcggcgttca actggatggt tctgaccacg tacatatgca ccatcatcat catcattctt
      541 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggagacc gacgtccaca
      601 tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt ctgaattgtg
      661 attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag agaatgaaaa
      721 gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt tatgattttc
      781 tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta atagaatgag
      841 gtcgaaaagt aaatcgcgcg ggtttgttac tgataaagca ggcaagacct aaaatgtgta
      901 aagggcaaag tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt t
//
