LOCUS       sequence-108115-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression plasmid, useful in conjunction with pUCXKT for 
            positive selection cloning and expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Prosser GA, Williams EM, Sissons JA, Walmsley KE, Parker MR, 
            Ackerley DF
  TITLE     A gain-of-function positive-selection expression plasmid that 
            enables high-efficiency cloning.
  JOURNAL   Biotechnol Lett. 2014 Sep 26.
  PUBMED    25257589
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett. 2014 Sep 26."
COMMENT     Sequence Label: pUCX
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(203..307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     375..393
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(431..453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     553..572
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      complement(647..674)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(766..852)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
       61 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      301 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
      361 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
      421 acggtgagaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      481 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
      541 ggcttaacta tgcggcatca gagcagattg tactgagagt gcacactagt cggatacata
      601 tttgaatgta tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt
      661 caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      721 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag
      781 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      841 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
      901 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
      961 tgccgccagg caaattctgt tttatcagac cgcttctgcg
//
