LOCUS       sequence-108116-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with internal stFRET inserted to measure
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Actinin-M-stFRET
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(221..244)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(245..496)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(287..308)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggcattgtt gatgtccgag accatcctgc cctcagaggg catgaaggca ggccggttgc
       61 tgagccgcag cttggtctgc agcgtgttga agttgatctc cagctggcac ttctcctgca
      121 ccttgggcgg cttgtgcagg cgccggtagt cccggaagtc ctccagcttc tgttgcatgg
      181 catgcatggt gttctcgggc acgcggccgc tgccacctcc gtggtgatga tggtggtgat
      241 gatgcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      301 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt
      361 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      421 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      481 cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatggcgttg tactccagct
      541 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      601 cca
//
