LOCUS       sequence-108121-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  positive selection cloning and expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Prosser GA, Williams EM, Sissons JA, Walmsley KE, Parker MR, 
            Ackerley DF
  TITLE     A gain-of-function positive-selection expression plasmid that 
            enables high-efficiency cloning.
  JOURNAL   Biotechnol Lett. 2014 Sep 26.
  PUBMED    25257589
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett. 2014 Sep 26."
COMMENT     Sequence Label: pUCXKT
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(127..144)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      297..383
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      475..502
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(577..596)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     696..718
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(756..774)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctgaaa gcttggctgt tttggcggat gagagaagat
      121 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      181 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      241 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      301 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      361 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      421 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
      481 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
      541 cattcaaata tgtatccgac tagtgtgcac tctcagtaca atctgctctg atgccgcata
      601 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      661 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
      721 ctcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
      781 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggg
//
