LOCUS       sequence-108122-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  positive selection cloning and expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Prosser GA, Williams EM, Sissons JA, Walmsley KE, Parker MR, 
            Ackerley DF
  TITLE     A gain-of-function positive-selection expression plasmid that 
            enables high-efficiency cloning.
  JOURNAL   Biotechnol Lett. 2014 Sep 26.
  PUBMED    25257589
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett. 2014 Sep 26."
COMMENT     Sequence Label: pUCXKT
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(309..314,315..330,331..337)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    341..365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             380..402
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(483..502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct
      121 gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
      181 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
      241 tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
      301 atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
      361 attcccctct agaaataatt ttgtttaact ttaagaagga gatatcatat gggatcctct
      421 agagtcgact aatagagctc gatggattgc acgcaggttc tccggccgct tgggtggaga
      481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      601 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
      901 tggacgaaga gcatca
//
