LOCUS       sequence-108125-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SNAP-AP fusion for tagging endogenous target for 
            SNAP-SiMPull.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Rodgers ML, Paulson J, Hoskins AA
  TITLE     Rapid isolation and single-molecule analysis of ribonucleoproteins 
            from cell lysate by SNAP-SiMPull.
  JOURNAL   RNA. 2015 May;21(5):1031-41. doi: 10.1261/rna.047845.114. Epub 2015 
            Mar 24.
  PUBMED    25805862
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.047845.114"; journalName: "RNA"; date: "2015-05"; 
            volume: "21"; issue: "5"; pages: "1031-41"
COMMENT     Sequence Label: pSNAPf-AP
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..555
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             574..618
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 aagcttcata tggacaaaga ctgcgaaatg aagcgcacca ccctggatag ccctctgggc
       61 aagctggaac tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct gggcaaagga
      121 acatctgccg ccgacgccgt ggaagtgcct gccccagccg ccgtgctggg cggaccagag
      181 ccactgatgc aggccaccgc ctggctcaac gcctactttc accagcctga ggccatcgag
      241 gagttccctg tgccagccct gcaccaccca gtgttccagc aggagagctt tacccgccag
      301 gtgctgtgga aactgctgaa agtggtgaag ttcggagagg tcatcagcta cagccacctg
      361 gccgccctgg ccggcaatcc cgccgccacc gccgccgtga aaaccgccct gagcggaaat
      421 cccgtgccca ttctgatccc ctgccaccgg gtggtgcagg gcgacctgga cgtggggggc
      481 tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc acgagggcca cagactgggc
      541 aagcctgggc tgggtgtcga cggcggttca gcgggcctga acgacatctt cgaggcccag
      601 aagatcgagt ggcacgagta aggatccccg ggttaattaa ggcgcgccag atctgtttag
      661 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
      721 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
      781 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
      841 aaaaattacg gctcctcgct gcggacctgc gagcagggaa a
//
