LOCUS       sequence-108131-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-11b
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(402..423)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(663..682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggagatatac catgggcagc agccatcatc atcatcatca cggcagcggc agcggctctg
       61 accaggaggc aaaaccttca actgaggact tgggggataa gaaggaaggt gaatatatta
      121 aactcaaagt cattggacag gatagcagtg agattcactt caaagtgaaa atgacaacac
      181 atctcaagaa actcaaagaa tcatactgtc aaagacaggg tgttccaatg aattcactca
      241 ggtttctctt tgagggtcag agaattgctg ataatcatac tccaaaagaa ctgggaatgg
      301 aggaagaaga tgtgattgaa gtttatcagg aacaaaccgg tggtcatatg gctagcaaag
      361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      541 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggt
//
