LOCUS       sequence-108141-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter containing a fragment of the 3'UTR
            of SPRED1 containing a miR-206 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atccaatgaa aataaaagat cctttattaa gcttaactgt ttaaacctat gatatcacgc
       61 acgcgtaagc ttgggcccct cgagggatac tctagagcgg ccgccctttt tttttttttt
      121 tttttaatga agaaacttgt ttaaaattgt aaaggaaaaa atgggaatgg gacggcaaaa
      181 tcttagcagc aaagtggtta aacaaattga aaatattaat gcacaaacat taaaatatta
      241 aagcatatat gttgcatata aaatacagta cagaaccagg agttgcacta tactgattag
      301 tgcttaacag aagaaatgat taaatttgtt cctcccagaa gtatatacac agttcatttc
      361 cacagcattt tcctatatag ccagcaagtt attttcttca gttattcaca ccttgatcaa
      421 acctgaatta taaacttagc acttacaaat atgaaaattc attcacaagg aaaaacagta
      481 tttccatttc accaataaaa attttgaaag ttaacagtct attctaggaa accaagttta
      541 gctgaaaact tcagggatga agatcatctg ttgtagcagc attcaaatat ataaacagta
      601 aaaataagac ttaaaactgc tgcctacagt gtcatgtttt ggatttagtt catccaattg
      661 atttttagta caaaactaga aataccctct tcttcataac atctatagtt atcaatatat
      721 tttcttcttt tcaatgtgaa ataatacttc aagatgatct atatacacaa tgttgtcagt
      781 tcaagctgtc atataaatat aaatgctttc ttaaaaaaag tattttacag acagatagtg
      841 ttatatacat
//
