LOCUS       sequence-108162-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM
  TITLE     SOX9 inhibits beta-TrCP-mediated protein degradation to promote 
            nuclear GLI1 expression and cancer stem cell properties.
  JOURNAL   J Cell Sci. 2015 Jan 27. pii: jcs.162164.
  PUBMED    25632159
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jan 27. pii: jcs.162164."
COMMENT     Sequence Label: pBABEPuro-HA-GLI1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..52
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 tgtggtggta cctagagacg ccatgtatcc ttatgacgtg cctgactatg ccagcctggg
       61 aggacctttc aactcgatga ccccaccacc aatcagtagc tatggcgagc cctgctgtct
      121 ccggcccctc cccagtcagg gggcccccag tgtggggaca gaaggactgt ctggcccgcc
      181 cttctgccac caagctaacc tcatgtccgg cccccacagt tatgggccag ccagagagac
      241 caacagctgc accgagggcc cactcttttc ttctccccgg agtgcagtca agttgaccaa
      301 gaagcgggca ctgtccatct cacctctgtc ggatgccagc ctggacctgc agacggttat
      361 ccgcacctca cccagctccc tcgtagcttt catcaactcg cgatgcacat ctccaggagg
      421 ctcctacggt catctctcca ttggcaccat gagcccatct ctgggattcc cagcccagat
      481 gaatcaccaa aaagggccct cgccttcctt tggggtccag ccttgtggtc cccatgactc
      541 tgcccggggt gggatgatcc cacatcctca gtcccgggga cccttcccaa cttgccagct
      601 gaagtctgag ctggacatgc tggttggcaa gtgccgggag gaacccttgg aaggtgatat
      661 gtccagcccc aactccacag gcatacagga tcccctgttg gggatgctgg atgggcggga
      721 ggacctcgag agagaggaga agcgtgagcc tgaatctgtg tatgaaactg actgccgttg
      781 ggatggctgc agccaggaat ttgactccca agagcagctg gtgcaccaca tcaacagcga
      841 gcactcacgg acgaagagtc tggcatgggg
//
