LOCUS       sequence-108172-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derivative of pKO-1 carrying the multiple cloning region from 
            M13mp19RF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Vimr ER, Lawrisuk L, Galen J, Kaper JB
  TITLE     Cloning and expression of the Vibrio cholerae neuraminidase gene 
            nanH in Escherichia coli.
  JOURNAL   J Bacteriol. 1988 Apr;170(4):1495-504.
  PUBMED    2832365
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1988-04"; volume: "170(4)"; pages: "1495-504"
COMMENT     Sequence Label: pCVD315
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..44)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     187..209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
       61 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
      121 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      181 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
      241 agaacagtcg gtacggctga ccatcgggtg ccagtgcggg agtttcgttc agcactgtcc
      301 tgctccttgt gatggtttac aaacgtaaaa agtctcttta atacctgttt ttgcttcata
      361 ttgttcagcg acagcttgct gtacggcagg caccagctct tccgggatca gcgcgacgat
      421 acagccgcca aatccgccgc cggtcatgcg tacgccacct ttgtcgccaa tcacagcttt
      481 gacgatttct accagagtgt caatttgcgg cacggtgatt tcgaaatcat cgcgcataga
      541 ggcatgagac tccgccatca actcgcccat acgtttcagg tcgccttgct ccagcgcgct
      601 ggcagcttca acggtgcggg cgttttcagt cagtatatga cgcacgcgtt ttgccacgat
      661 cgggtccagt tcatgcgcaa cagcgttgaa ctcttcaatg gtgacatcac gcagggctgg
      721 ctgctggaag aaacgcgcac cggtttcgca ctgttcacga cgggtgttgt attcgctgcc
      781 aaccagggta cgtttgaagt tactgttgat gatgacgaca gccacac
//
