LOCUS       sequence-108174-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing GFP from BLBP promoter which is a neural stem cell 
            (radial glial cells) specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Shariati SA, Lau P, Hassan BA, Muller U, Dotti CG, De Strooper B, 
            Gartner A
  TITLE     APLP2 regulates neuronal stem cell differentiation during cortical 
            development.
  JOURNAL   J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77. doi: 10.1242/jcs.122440. 
            Epub 2013 Jan 23.
  PUBMED    23345401
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-03-1"; volume: "126(Pt 5):1268-77. doi"; pages: "
            10.1242/jcs.122440. Epub 2013 Jan 23"
COMMENT     Sequence Label: BLBP-GFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(82..795,796..798,799..801))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(85..795,796..798,799..801))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(127..148)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     476..495
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     735..756
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggagt
       61 gcggccgcta ttatcggtac cttacttgta cagctcgtcc atgccgagag tgatcccggc
      121 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      181 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      241 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      301 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      361 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      421 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      481 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      541 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
      601 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      661 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      721 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      781 ctcctcgccc ttgctcacca tggtggcgac cggtggcgcg ccaggcagga actggaggaa
      841 ctcgggtctt acc
//
