LOCUS       sequence-108178-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-ER-5
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..844
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tttagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatgc tgctatccgt
       61 gccgttgctg ctcggcctcc tcggcctggc cgtcgccgca ccggtcgcca ccatggtgtc
      121 taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
      181 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
      241 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
      301 taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca tccccgattt
      361 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
      421 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
      481 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
      541 ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaaccga
      601 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag
      661 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact
      721 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc
      781 cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta
      841 caagtccgga aaggacgagc tgtaagaatt ctgcagtcga cggtaccgcg ggcccg
//
