LOCUS       sequence-108181-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mTagRFP-T-Mito-7
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..111
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..876
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTETLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 agcgagctgg tttagtgaac cgtcagatcc gctagcatgt ccgtcctgac gccgctgctg
       61 ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg
      121 ttgggggatc caccggtcgc caccatggtg tctaagggcg aagagctgat taaggagaac
      181 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
      241 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
      301 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
      361 ttcatcaacc acacccaggg catccccgat ttctttaagc agtccttccc tgagggcttc
      421 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      481 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
      541 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gaccctgtac
      601 cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc
      661 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
      721 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
      781 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
      841 gggcacaaac ttaatggcat ggacgagctg tacaagtaag cggccgcgac tc
//
