LOCUS       sequence-108185-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..820)
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcataa cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagagtcgc ggccgcctat cattacttgg cctgcctcgg cagctcagaa tgcgcttcgg
      181 cgtgctcatg cagattaaca ttactgtaat ctttgtcgtg gcttttaatc tcaatgtggt
      241 ggtccacaaa gtgatagtct ggcaactgga caaccttctt agctttataa gtagttttga
      301 agtcacatcg gtaatggcca cctccttcaa gcgacagagc catgttaaca tcacccttca
      361 gcactccatc acgcacatac aatttctcag tggatggctc ccatttcaca gtcctcttct
      421 gcataactgg accattggca ggaaagttca caccatcaaa tcgaatttca tagatataac
      481 agtcaccatc cagggttatg tcgtttgtcg cgttacaaat gcccccgtct tcgtaattca
      541 tgcttcgttc ccaagagtag ccctcaggaa acgactgctt gaaatagtct actatatttt
      601 ctgggtattt ggcgaatacc ctgttgccgt aacagaacac agttgtcaag atgtcatagg
      661 cgaaaggcag aggtccgcct tctttgactt taaggtccat actctgtttt ccctcgaaag
      721 gcttcccaag gccaactcct tcaatcgcga acgggtgtcc atttacagcg ccttccatac
      781 gcagcttgat cttcatgtct ggtttaatca cactcaccat ggtggcgacc ggtggatcct
      841 gagtgcgtgg gattccagcc acgatttctg attcaattcc acagtggttc tctcctctga
//
