LOCUS       sequence-108186-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing mCherry from BLBP promoter which is a neural stem cell 
            (radial glial cells) specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Shariati SA, Lau P, Hassan BA, Muller U, Dotti CG, De Strooper B, 
            Gartner A
  TITLE     APLP2 regulates neuronal stem cell differentiation during cortical 
            development.
  JOURNAL   J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77. doi: 10.1242/jcs.122440. 
            Epub 2013 Jan 23.
  PUBMED    23345401
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-03-1"; volume: "126(Pt 5):1268-77. doi"; pages: "
            10.1242/jcs.122440. Epub 2013 Jan 23"
COMMENT     Sequence Label: BLBP-mCherry
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(87..797)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASYERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(365..384)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     634..652
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      794..803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggagtgcggc cgctattatc ggtaccttac ttgtacagct cgtccatgcc gccggtggag
      121 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
      181 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
      241 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
      301 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cgtaggaggc ctcccagccc
      361 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
      421 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
      481 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
      541 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
      601 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
      661 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
      721 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
      781 tcgcccttgc tcaccatggt ggcgaccggt ggcgcgccag gcaggaactg gaggaactcg
      841 ggtcttaccc accaccgtaa ttcacaaaaa ccctttgcac ctcagcagaa gctgctgcct
      901 tcttatttag agaagaagcg ggcaaaa
//
