LOCUS       sequence-108189-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-tagged mouse beta-TrCP residues 1-247.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM
  TITLE     SOX9 inhibits beta-TrCP-mediated protein degradation to promote 
            nuclear GLI1 expression and cancer stem cell properties.
  JOURNAL   J Cell Sci. 2015 Jan 27. pii: jcs.162164.
  PUBMED    25632159
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jan 27. pii: jcs.162164."
COMMENT     Sequence Label: pcDNA3.1 myc-m-beta-TrCP F-box (1-247)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..44
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             51..80
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             87..116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(159..192)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcttgccac catggagcaa aagctcattt ctgaagagga cttgaatgga gaacagaaat
       61 tgatcagtga ggaagacctc aacggtgagc agaagttaat atccgaggag gatcttaata
      121 gtagcggccg cactagtctc gaaggggggc cccatatgat aacttcgtat agcatacatt
      181 atacgaagtt atctggaatt ccccgggctc gagactccat ggggcatatg gacccggcag
      241 aggcggtgct gcaggagaaa gcgcttaagt ttatgaattc ctcagagaga gaagactgta
      301 ataatggcga accccctagg aagataatac cagagaagaa ttcacttaga cagacttaca
      361 acagctgtgc caggctttgc ataaaccaag agacagtatg tctaacaagc actgctatga
      421 agactgaaaa ttgtgtggcc aaagccaaac ttgccaatgg cacttccagc atgattgtgc
      481 ccaagcagcg gaaactctca gcaagctatg agaaggaaaa ggagctgtgt gtcaagtatt
      541 ttgagcagtg gtcagagtct gatcaagtgg aatttgtaga acaccttata tcccaaatgt
      601 gtcactacca gcatgggcac atcaactcct acctaaaacc tatgctgcag agggatttca
      661 taactgcact gccagcacgg ggtctggacc acatcgctga gaacattctg tcatacttgg
      721 acgccaagtc actgtgtgct gctgagctcg tgtgcaagga atggtaccgc gtgacgtcgg
      781 acggcatgct gtggaaaaag ctcatcgaga ggatggtcag gacggactct ctgtggcgag
      841 gcctggc
//
