LOCUS       sequence-108191-        5522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitin promoter driving synthetic xylanase (sXynA) gene. Can be 
            used as a reporter of promoter activity in plants by replacing Ubi 
            promoter with sequence of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM
  TITLE     A synthetic xylanase as a novel reporter in plants.
  JOURNAL   Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4.
  PUBMED    12845475
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003-09"; volume: "22(2)"; pages: "135-40. Epub 2003 
            Jul 4"
COMMENT     Sequence Label: pUbiSXR
FEATURES             Location/Qualifiers
     source          1..5522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2345..2364
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2393..4384
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     promoter        complement(join(5521..5522,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(5522,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatctga tagcttgcat gcctgcagtg
     2401 cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa
     2461 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata
     2521 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg
     2581 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg
     2641 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa
     2701 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg
     2761 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta
     2821 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag
     2881 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg
     2941 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta
     3001 acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg
     3061 catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc
     3121 cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg
     3181 gcctcctcct cctctcacgg cacggcagct acgggggatt cctttcccac cgctccttcg
     3241 ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac
     3301 ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc
     3361 acctccgctt caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga
     3421 tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag
     3481 atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc
     3541 agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct
     3601 agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg
     3661 tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca
     3721 tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga
     3781 gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc
     3841 catacatatt catagttacg aattgaagat gatggatgga aatatcgatc taggataggt
     3901 atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt
     3961 gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt
     4021 ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca
     4081 tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg
     4141 ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt
     4201 gagtacctat ctattataat aaacaagtat gttttataat tattttgatc ttgatatact
     4261 tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta
     4321 tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct
     4381 gcaggtcgac tctagaggat cctaaagcaa gcaagagcaa taaacaatgg ctagcaacgg
     4441 gaagaagttc accgtcggga acggccaaaa ccaacacaag ggcgtcaacg acggcttcag
     4501 ctacgagatt tggctcgaca acaccggtgg caacggctcc atgaccctcg gtagcggcgc
     4561 aacgttcaag gcagagtgga acgcagccgt gaaccgcggc aacttcctgg cacggagggg
     4621 cctcgacttc gggagccaaa agaaggccac cgactacgac tacatcggcc tggactacgc
     4681 agccacctac aagcagaccg ccagcgcaag cggcaactcc aggctctgcg tgtacggctg
     4741 gttccagaac aggggtctga acggcgtgcc actcgtcgag tactacatca tcgaggactg
     4801 ggtcgactgg gtgccagacg cacaaggcaa gatggtcacc atcgacgggg cacagtacaa
     4861 gatcttccag atggaccaca ctggcccaac catcaacggt ggcagcgaga ccttcaagca
     4921 atacttctcc gtcaggcaac agaagaggac ctccgggcac atcaccgtct ccgaccactt
     4981 caaggagtgg gccaagcaag gctggggtat cggcaacctc tacgaggtgg cactgaacgc
     5041 cgaggggtgg caaagctccg gtgtggctga cgtcaccctg ctggacgtgt acaccactcc
     5101 aaagggctcg agcccagcca cctcagccgc accacgctga agcttacgta tgatccccgg
     5161 gctgcgagga gtctggtggc aactaagccg tcatcgtcat atatagcctc gtttaattgt
     5221 tcatctctga ttcgatgatg tctcccacct tgtttcgtgt gttcccagtt tgttcatcgt
     5281 cttttgattt taccggccgt gctctgcttt tgtttttgtt tcacctgatc tctctctgac
     5341 ttgatgtaag agtggtatct gctacgacta tatgttgttg ggtgaggcat atgtgaatga
     5401 aatctatgaa agctccggct atatatattt atacaaaggg tatgagatgg atgtgaatcc
     5461 tagagcatat gtgtccaaca atcaattcgt cgagggggag cttcagctgc tcgatcgagt
     5521 tc
//
