LOCUS       sequence-108198-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, 
            Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK V5-LUC Neo (w623-2)
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(107..148)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(160..184)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(239..749)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(302..320)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaatgcc
       61 aagctttttg caaaagccta gggactgaat tggttcccat ggatcccgta gaatcgagac
      121 cgaggagagg gttagggata ggcttacctt ccatggtgga gcctgctttt ttgtacaaac
      181 ttgttgatat ctgcagaatt ccaccacact ggactagtcg acgaaccggt ggatccccct
      241 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
      301 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
      361 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
      421 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
      481 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
      541 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
      601 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
      661 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
      721 tgccttggaa aaggcgcaac cccaaccccg tggaattcga tatcaaagct
//
