LOCUS       sequence-108201-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad host range plasmid for constitutive expression of mCherry. 
            Designed for expression in Pseudomonas aeruginosa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  TITLE     Franklin lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMF440
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(283..300)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      453..539
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      632..659
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttagacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtagaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      301 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
      361 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      421 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
      481 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      541 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg aggggtggcg ggcaggacgc
      601 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
      661 gcgtttctac aaactctttt g
//
