LOCUS       sequence-108207-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 
            (incorrect orientation for expression off of pTTQ181 promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Jerse AE, Yu J, Tall BD, Kaper JB
  TITLE     A genetic locus of enteropathogenic Escherichia coli necessary for 
            the production of attaching and effacing lesions on tissue culture 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43.
  PUBMED    2172966
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1990-10"; volume: "87(20)"; pages: 
            "7839-43"
COMMENT     Sequence Label: pCVD437
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2..24)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     124..143
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      complement(258..285)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(377..463)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     616..633
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
       61 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      121 atgcggcatc agagcagatt gtactgagag tgcaccataa attccctgct ttcctgatgc
      181 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
      241 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
      301 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
      361 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      421 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct
      481 actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
      541 ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga
      601 ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa
      661 cagggaattt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
      721 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      781 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      841 agggttttcc cagtcacgac gttgtaa
//
