LOCUS       sequence-108211-        5573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing mCherry from BLBP promoter which is a neural stem cell 
            (radial glial cells) specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Shariati SA, Lau P, Hassan BA, Muller U, Dotti CG, De Strooper B, 
            Gartner A
  TITLE     APLP2 regulates neuronal stem cell differentiation during cortical 
            development.
  JOURNAL   J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77. doi: 10.1242/jcs.122440. 
            Epub 2013 Jan 23.
  PUBMED    23345401
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-03-1"; volume: "126(Pt 5):1268-77. doi"; pages: "
            10.1242/jcs.122440. Epub 2013 Jan 23"
COMMENT     Sequence Label: BLBP-mCherry
FEATURES             Location/Qualifiers
     source          1..5573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             524..535
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      1782..1791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1788..2498
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASYERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1933..1951)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2201..2220
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2597..2616)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2662..2717
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2716..2735)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3076..3092)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3076..3092)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3089..3111)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3100..3116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3124..3130,3131..3148,3149..3154))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3169..3190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3249..3444
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3295..3430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3450..3584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3487..3506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3541..3560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3652..3669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3823..4411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3903..3922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4582..5373,5374..5442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5205..5224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5443..5547)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacagc acagcggaaa gggaaaaaaa aaccccaact ttaaagtagg aaaatattta
       61 catagtcact gcatgaacgg tattcaaaac ccagaaaatc acattaaaaa aatgttcagt
      121 gtccttagta gtgaggggaa tgcaaatttg tgtttgaaag gttgctggtt tccaagagat
      181 cctttgagaa cagtgtggta gctgctgcta aagaatcagg caaagccaga agttgctcac
      241 atttaggagg cttgtgtgct gggggttggg aggatgactg ctaaatactg gggaagcaaa
      301 atgagctaag tctttacagc aactgtcatt tgtgagagta aaccattaga aacggacggc
      361 aggtttgtgg tagattccac aaggtggtgg aactagtcgc atatgtagtt cagacttgaa
      421 ggcacacacg tatggggaca gttctggtgt ttacgataaa caggtatgct gccatccagc
      481 tagcacagca ctaggctaaa gcgtactgag cccttgtctt ccgtgggagc tgcagagtgg
      541 gatgcatgcg ttgtgagctg aggctcaagc tgcgctggca gaagagcagg ggttgccttg
      601 tcagactcca gggtctcttt ctctctgagc ctgggaaagt gccactttat tggatctata
      661 aagccggggg gggggggggg ggaggaatct caaggtgaag aggaagttca cagacccctc
      721 taacgcctct attagaacct tccagctatt ctctcatact tgtacactga gctggcacac
      781 agtataggca agttctattc gcatcacccc tctagttcct gtctccctgg ttatgcaagc
      841 ctcatattta ggtagatgtg accttaggaa accaaaatat cctttaagat cttactaact
      901 ggttgcctgt tcagcttttc cacattgatc ctgtagcccc ctcgaggagg tgaaggaaaa
      961 aaatctcctc tttgtttctc taactcatta atgaatttta agggcactct gtaaggttcc
     1021 tttcccattc tggtctggtt cgtacattct gagaaacaca ctgtgtttgt gttgagagtt
     1081 ggctccctag ctacactgtc tgtcacattg atgctctgag tagggacagg gttcatctag
     1141 gaaatatatt ttcactcaca ctctgtatct tttcctagtt tggcatattc tagtctgcat
     1201 ttggctctct gtttaaatat aaaagaaaac taaaacacac ccttcagacg cctatgtctg
     1261 aaaaatctgg catttccgtg ggtttttctt taaggaggcc ttcatttgta accaacacca
     1321 tgctctcctt aaggaaatca atctcaatgc cctattatcc ttcccttttc tttcctccca
     1381 gtttgaggct gcagttgcct ttttttttct tatcccctgc tgaacctgaa aaaccctctc
     1441 ttttctacag ttttctgttc ccaggccccg ctgacttcct ttagagcatg gggggggggg
     1501 ggatcaggat tgtgatgtgt gaactgggag gatcttgacc tactccgcta acccagtggc
     1561 ctgagcaaat cacaaggagg attggagcca tctgcccagc ccctccccca cggcagcctg
     1621 ctggaaagag acaagttagt cattcaaatg attggctttt tgcccgcttc ttctctaaat
     1681 aagaaggcag cagcttctgc tgaggtgcaa agggtttttg tgaattacgg tggtgggtaa
     1741 gacccgagtt cctccagttc ctgcctggcg cgccaccggt cgccaccatg gtgagcaagg
     1801 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1861 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1921 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1981 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     2041 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     2101 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     2161 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     2221 ccatgggctg ggaggcctcc tacgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     2281 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     2341 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     2401 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     2461 gccactccac cggcggcatg gacgagctgt acaagtaagg taccgataat agcggccgca
     2521 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     2581 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     2641 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     2701 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     2761 atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg
     2821 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     2881 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     2941 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     3001 ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcagcccaag
     3061 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3241 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3301 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3361 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3421 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     3481 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3541 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     3601 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3721 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3781 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3841 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3901 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3961 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     4021 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4081 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4141 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4201 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4261 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4321 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4381 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4441 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4501 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4561 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4621 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4681 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4741 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4801 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4861 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4921 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4981 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5041 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5101 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5161 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5221 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5281 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5341 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5401 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5461 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5521 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
