LOCUS       sequence-108221-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  prey plasmid for an adapted two-hybrid system for C. albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Stynen B, Van Dijck P, Tournu H
  TITLE     A CUG codon adapted two-hybrid system for the pathogenic fungus 
            Candida albicans.
  JOURNAL   Nucleic Acids Res. 2010 Oct;38(19):e184. doi: 10.1093/nar/gkq725. 
            Epub 2010 Aug 17.
  PUBMED    20719741
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-10"; volume: "38(19):e184. doi"; pages: " 
            10.1093/nar/gkq725. Epub 2010 Aug 17"
COMMENT     Sequence Label: pC2HP
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             207..227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             378..443
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ataataaagt atataataga tcatcaagta tacgtaatct ccccaaaaaa aaaattgcat
       61 aaaataaaat ctataaacct tttttagatt tttagttgtt ccttttttct ttctattctt
      121 caacttccaa gtgttgtcac tttcttcctg ttaactatat ttaaaagtaa ataccctccc
      181 cagaaaactc cggaatgggt gctcctccaa aaaagaagag aaaggtagct ccgcggtctc
      241 cagggcccgg tttcacccca cacgattctg ctccatacgg tgctttggat atggctgatt
      301 tcgaattcga acaaatgttc accgatgctt tgggtattga tgaatacggt ggtgctagcg
      361 gtgctggtgg tgttggtgac tacaaagacc atgacggtga ttataaagat catgacatcg
      421 attacaagga tgacgatgac aagggagcag gaggcgtcgg aaggcctccc gggaggcgcg
      481 ccacgcgtag tacttaagat cctgaagacg ggatcgttcg aagagtgaaa ttctggaaat
      541 ctggaaatct ggttttgtat tcttgttatt cttctttttg ttattacata tataacttgt
      601 tactttttta aaaaaatctt tgtttatttt ataaatatat aaaactaaat ttaagaaaaa
      661 gagaaaaatg ttttatttga gagattgaaa ttttacttga atttagctta gcttttataa
      721 agtattatta tgtaaaaaaa caaaacaaat atacattaaa aagttaagac tataaaatag
      781 ccacccaagg catttctata tcttgttgtt gttgttttca tcttctgtat cagaggaact
      841 tattttatta tt
//
