LOCUS       sequence-108222-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 
            0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best 
            EHEC probe for detection of this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Levine MM, Xu JG, Kaper JB, Lior H, Prado V, Tall B, Nataro J, Karch
            H, Wachsmuth K
  TITLE     A DNA probe to identify enterohemorrhagic Escherichia coli of 
            O157:H7 and other serotypes that cause hemorrhagic colitis and 
            hemolytic uremic syndrome.
  JOURNAL   J Infect Dis. 1987 Jul;156(1):175-82.
  PUBMED    3298451
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Infect 
            Dis."; date: "1987-07"; volume: "156(1)"; pages: "175-82"
COMMENT     Sequence Label: pCVD419
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        81..183
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             184..843
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(250..269)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac
       61 ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      121 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      181 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      241 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      301 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      361 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      421 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      481 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      541 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      601 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
      661 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
      721 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
      781 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
      841 taatt
//
