LOCUS       sequence-108223-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  containing the bottom toxin fragment from a HindIII digest of V. 
            cholerae 395 chromosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Kaper JB, Lockman H, Baldini MM, Levine MM
  TITLE     A Recombinant Live Oral Cholera Vaccine
  JOURNAL   Nature Biotechnology 2, 345 - 349 (1984)
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Biotechnology 2, 345 - 349 (1984)"
COMMENT     Sequence Label: pCVD15
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        81..183
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(250..269)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac
       61 ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      121 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      181 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      241 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      301 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      361 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      421 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      481 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      541 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      601 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
      661 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
      721 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
      781 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcgg
//
