LOCUS       sequence-108229-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with 
            CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.F3
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(119..125,126..143,144..149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccttccatac gaactttgaa acgcatgaac tctttgataa cgtcttcgga ggaagccatc
       61 tagtatttct cctctttctc tagtatgtgt gaaattgtta tccgctcaca attccacaca
      121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggtct cattttgctc
      301 gagaaacttc tgtggcttga cagataaata actgcagtag tcggtgcgta ctaattgttt
      361 ggtcgtgtct gaaaaatctt gaattttcag aaaagaataa gccccaaatg tcagtgatgg
      421 tagtagcagt actcccctac gattttagat actttagaga gcccaccttc agaatcggaa
      481 ggaggataat tttgtaaagc ccttctgttt tttctcttgc ataacttata tttccacatc
      541 aaaaagtagt gtgctaagaa aaaggagacg agaaaaagga ttacggcact ctctgcatct
      601 agacatatac caaaagttgg gtttgctcac gaaaatacca taattgtggt gtcaaaaaaa
      661 tcctgcctca taataccact gcagcaattg tggatgacta aaaaataact tgcattccac
      721 gatgttattt tactttataa agcacctgca attttttttt ttgtattaac tcatcgagta
      781 tgtctgatgg atacgcggcc gctgcagtcc ggcaa
//
