LOCUS       sequence-108231-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derivative of pACYC184 M1uI linkers inserted into EcoRV site 
            inactivating Tc resistance gene. Now Tcs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Donnenberg MS, Calderwood SB, Donohue-Rolfe A, Keusch GT, Kaper JB
  TITLE     Construction and analysis of TnphoA mutants of enteropathogenic 
            Escherichia coli unable to invade HEp-2 cells.
  JOURNAL   Infect Immun. 1990 Jun;58(6):1565-71.
  PUBMED    2160428
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "1990-06"; volume: "58(6)"; pages: "1565-71"
COMMENT     Sequence Label: pCVD433
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(388..407)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
       61 ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
      121 ttgcgggatg acgcgtcatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
      181 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
      241 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
      301 tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca
      361 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      421 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
      481 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
      541 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
      601 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
      661 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
      721 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
      781 gcctgtcgct tgcggtattc ggaatcttgc acgcc
//
