LOCUS       sequence-108244-        8867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL
FEATURES             Location/Qualifiers
     source          1..8867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             992..1369
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1388..1397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1539..1557)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1807..1826
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2642..2653)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4819..4836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4825..5049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5182..5201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5333..5380
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5388..6410
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5388..6410
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6540..6661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6577..6596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6631..6650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6710..6726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6710..6726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6723..6745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6734..6750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6758..6764,6765..6782,6783..6788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6803..6824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6941..6958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7112..7700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7192..7211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7871..8662,8663..8731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8494..8513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8732..8836)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttcgccac catgccagag ccagcgaagt ctgctcccgc
     1021 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     1081 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     1141 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     1201 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     1261 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     1321 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt
     1381 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc aggtgagtac
     1981 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     2041 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     2101 gaaaagacaa tctttttgtt gtcaagctgt ttgactcatg cacccgctac tcttcaacct
     2161 tgcagactaa ttaatggcaa ctctgacaga tgtggcggga ggaaagctga ggtgggagta
     2221 ctgctcagag ctacaactct aggagtaggg actccaagcc tagagctcca agagagggtg
     2281 aagggcaggg agaaaattat agtaatagtt gcaatgagtc acttgcttct agatcaagat
     2341 cagagactct gctctccgtg ttcacagcgg accttgattt aatgtcatac aattaaggca
     2401 cgcggtgaat gccaagagcg gagcctacgg ctgcacttga aggacatccg agagaagtta
     2461 ggaagggtgg ggagaaacaa ttctagaatg aacccatcct gtgcgacaca aggacccctc
     2521 gcattgattc aaaatcttta gaatacaaga cagtcaaatg tttttcagcg agaatctggg
     2581 aaatccgcat gcatactccc aaggggaata ttagaggttt cattgacagc tgtcaggaga
     2641 gctgtagttc ccattggtgc aaacggtcta agagatggca ctcagagagt gctggccttt
     2701 tccaaatact agtcatatgc ggccgcagct tgaggtgtga caggcttgag atctggccat
     2761 acacttgagt gacaatgaca tccactttgc ctttctctcc acagctgccc ggcgcctaca
     2821 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2881 acgaacgcgc cgagggccgc cactccaccg gtggcatgga cgagctgtac aagtctgacc
     2941 ttgatatcca gcacagtggc ggccgctcga gtagccatgg cttcccgccg gcggtggcgg
     3001 cgcaggatga tggcacgctg cccatgtctt gtgcccagga gagcgggatg gaccgtcacc
     3061 ctgcagcctg tgcttctgct aggatcaatg tgtagtaatg agtttaaggc gcgcggctct
     3121 agagctgaag cccgatattc aggatcttct tcaagacttt ctacttagaa cctgccgtgt
     3181 tatcgagctt acctattgtt tttttccaaa tgattatttt tgttagttta catacagttt
     3241 agtgcctaga aaatgtgcct ttgtttttca tatacactcc aaactggagg tgtgacctac
     3301 cctccccaca ttttgcacag tgcctttaca gatttctgga ctgggaaaga ggacttgtgt
     3361 agttctgagt gctgaatgga ggtggatgtt gtcactcaag atgacttgtt gtggattcca
     3421 ggaagggggt tttgttcaag gcagctgtcc ccacatctga catcatcttc aaaaccacat
     3481 tttaaacttt tgggtaatta gattcccaac ttggaccttc caagaagaac actactgcct
     3541 aacagcaatg ggtgacaata gcagggtgtg ccttattttg atagctaggt aactgttctt
     3601 taagattatt ctccgtttgt aagcactact gactcccgct gtatttaaga tgctggtgaa
     3661 gagctcatgc tcttatgtta gatatgacta tgtttacttt cttgttcttg ttgtatatca
     3721 ctcctaaaat agtattttaa ccagaaataa ccctttaaga agtttttata gaactatgac
     3781 caaagtttct attttgccaa aagttaagta caaataaatt gccccaatag atacattcaa
     3841 ataagtgcca aatgtaactc aaggtattct ttaagaaaaa tgttaatact gtgagagtga
     3901 gccttcaata tgtcggcagt ccatcctgtg aaatgctata gagaaggcta gtcattctga
     3961 tttggtttct tctttagagc tatggaggtg ggctcatatc acacctgtag acaagatact
     4021 tagccagcag tttgtagact gagctaggct gtggtctcta gtgcaatgtg agcagtagcc
     4081 gtgcagcctc accaaggcat cagtctgcac acagttccag aagactgtgt actgcttgta
     4141 ctcatctctg tcttatgctt gaggtaagcc accggtcact cgtagaggat agctaaccag
     4201 agatgcctgg tgctcttaga atgtagccat tgctgtctgg gatagtttca ttgtttatat
     4261 gagactttca tcaaggcttg gttcaaagac aggagttcag gcatgggtgg aattgaggaa
     4321 gtggtgaagg gaaagctgct tgcctatggc agtgccacta gaatgtcctg aaatctgtgt
     4381 ttgtgtgtaa agcagcatta ctcaagatgt catagaatct ggttctagtt ttttgttttt
     4441 gttttgtttt gttttttgag acagggtttc tctgtatagc ccttgctgtc ctggaactca
     4501 ctttgtagac caggctggct cgaactcaaa aatctgcctg cctctgcctc ccaagtgcag
     4561 ggattaaagg cgtgtgccac caacgcccaa ctggttctag ttttttatct gttcccctaa
     4621 cattactgaa acaatacaga tgttgaagat atgccttgcc gcatcagcct agggcttctg
     4681 tcctggtctg acctcacaac tttggtgctg tctcctcata ttctgtccca tgtaactcag
     4741 tggctcctgt attaacacag tgatacctcg cttctctgtg catttagctg ggagggcccg
     4801 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4861 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4921 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4981 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5041 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5101 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5161 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5221 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5281 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc
     5341 tattccgaag ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac
     5401 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     5461 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     5521 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     5581 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     5641 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     5701 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     5761 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     5821 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     5881 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     5941 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     6001 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     6061 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     6121 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     6181 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     6241 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     6301 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     6361 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac
     6421 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6481 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6661 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
