LOCUS       sequence-108247-        8856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with one seed site 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d3
FEATURES             Location/Qualifiers
     source          1..8856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             992..1369
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1388..1397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1539..1557)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1807..1826
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2642..2653)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4808..4825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4814..5038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5171..5190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5322..5369
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5377..6399
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5377..6399
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6529..6650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6566..6585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6620..6639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6699..6715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6699..6715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6712..6734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6723..6739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6747..6753,6754..6771,6772..6777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6792..6813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6930..6947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7101..7689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7181..7200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7860..8651,8652..8720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8483..8502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8721..8825)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttcgccac catgccagag ccagcgaagt ctgctcccgc
     1021 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     1081 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     1141 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     1201 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     1261 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     1321 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt
     1381 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc aggtgagtac
     1981 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     2041 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     2101 gaaaagacaa tctttttgtt gtcaagctgt ttgactcatg cacccgctac tcttcaacct
     2161 tgcagactaa ttaatggcaa ctctgacaga tgtggcggga ggaaagctga ggtgggagta
     2221 ctgctcagag ctacaactct aggagtaggg actccaagcc tagagctcca agagagggtg
     2281 aagggcaggg agaaaattat agtaatagtt gcaatgagtc acttgcttct agatcaagat
     2341 cagagactct gctctccgtg ttcacagcgg accttgattt aatgtcatac aattaaggca
     2401 cgcggtgaat gccaagagcg gagcctacgg ctgcacttga aggacatccg agagaagtta
     2461 ggaagggtgg ggagaaacaa ttctagaatg aacccatcct gtgcgacaca aggacccctc
     2521 gcattgattc aaaatcttta gaatacaaga cagtcaaatg tttttcagcg agaatctggg
     2581 aaatccgcat gcatactccc aaggggaata ttagaggttt cattgacagc tgtcaggaga
     2641 gctgtagttc ccattggtgc aaacggtcta agagatggca ctcagagagt gctggccttt
     2701 tccaaatact agtcatatgc ggccgcagct tgaggtgtga caggcttgag atctggccat
     2761 acacttgagt gacaatgaca tccactttgc ctttctctcc acagctgccc ggcgcctaca
     2821 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2881 acgaacgcgc cgagggccgc cactccaccg gtggcatgga cgagctgtac aagtctgacc
     2941 ttgatatcca gcacagtggc ggccgctcga gtagccatgg cttcccgccg gcggtggcgg
     3001 cgcaggatga tggcacgctg cccatgtctt gtgcccagga gagcgggatg gaccgtcacc
     3061 ctgcagcctg tgcttctgct aggatcaatg tgtagataag aatgggcccc accgatgaaa
     3121 ctgaagcccg atattcagga tcttcttcaa gactttctac ttagaacctg ccgtgttatc
     3181 gagcttacct attgtttttt tccaaatgat tatttttgtt agtttacata cagtttagtg
     3241 cctagaaaat gtgcctttgt ttttcatata cactccaaac tggaggtgtg acctaccctc
     3301 cccacatttt gcacagtgcc tttacagatt tctggactgg gaaagaggac ttgtgtagtt
     3361 ctgagtgctg aatggaggtg gatgttgtca ctcaagatga cttgttgtgg attccaggaa
     3421 gggggttttg ttcaaggcag ctgtccccac atctgacatc atcttcaaaa ccacatttta
     3481 aacttttggg taattagatt cccaacttgg accttccaag aagaacacta ctgcctaaca
     3541 gcaatgggtg acaatagcag ggtattttga tagctaggaa actgttcttt aagattattc
     3601 tccgtttgta agcactactg actcccgctg tatttaagat gctggtgaag agctcatgct
     3661 cttatgttag atatgactat gtttactttc ttgttcttgt tgtatatcac tcctaaaata
     3721 gtattttaac cagaaataac cctttaagaa gtttttatag aactatgacc aaagtttcta
     3781 ttttgccaaa agttaagtac aaataaattg ccccaataga tacattcaaa taagtgccaa
     3841 atgtaactca aggtattctt taagaaaaat gttaatactg tgagagtgag ccttcaatat
     3901 gtcggcagtc catcctgtga aatgctatag agaaggctag tcattctgat ttggtttctt
     3961 ctttagagct atggaggtgg gctcatatca cacctgtaga caagatactt agccagcagt
     4021 ttgtagactg agctaggctg tggtctctag tgcaatgtga gcagtagccg tgcagcctca
     4081 ccaaggcatc agtctgcaca cagttccaga agactgtgta ctgcttgtac tcatctctgt
     4141 cttatgcttg aggtaagcca ccggtcactc gtagaggata gctaaccaga gatgcctggt
     4201 gctcttagaa tgtagccatt gctgtctggg atagtttcat tgtttatatg agactttcat
     4261 caaggcttgg ttcaaagaca ggagttcagg catgggtgga attgaggaag tggtgaaggg
     4321 aaagctgctt gcctatggca gtgccactag aatgtcctga aatctgtgtt tgtgtgtaaa
     4381 gcagcattac tcaagatgtc atagaatctg gttctagttt tttgtttttg ttttgttttg
     4441 ttttttgaga cagggtttct ctgtatagcc cttgctgtcc tggaactcac tttgtagacc
     4501 aggctggctc gaactcaaaa atctgcctgc ctctgcctcc caagtgcagg gattaaaggc
     4561 gtgtgccacc aacgcccaac tggttctagt tttttatctg ttcccctaac attactgaaa
     4621 caatacagat gttgaagata tgccttgccg catcagccta gggcttctgt cctggtctga
     4681 cctcacaact ttggtgctgt ctcctcatat tctgtcccat gtaactcagt ggctcctgta
     4741 ttaacacagt gatacctcgc ttctctgtgc atttagctgg gagggcccgt ttaaacccgc
     4801 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4861 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5041 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5281 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     5341 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     5401 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5461 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5521 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5581 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     5641 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5701 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     5761 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     5821 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     5881 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     5941 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6001 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6061 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6121 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6181 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6241 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6301 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6361 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     6421 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6481 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6661 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6901 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7021 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7921 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7981 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8041 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8101 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8161 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8221 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8281 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8341 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8401 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8461 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8521 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8581 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8641 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8701 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8761 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8821 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
