LOCUS       sequence-108251-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PIq driving expression GalS-T, the Fucose inducible chimera
            with the TAN DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[GalS-T]
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        472..549
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(569..588)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 caacaaattg ataagcaatg cttttttata atgccaactt agtataaaaa agctgaacga
       61 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      121 acataatact gtaaaacaca acatatgcag tcactatgaa tcaactactt agatggtatt
      181 agtgacctgt aacagagcat tagcgcaagg tgatttttgt cttcttgcgc taattttttg
      241 tcatcaaacc tgtcgcgcac tctagctcta ggctactcag ctatctagac atatgaattc
      301 ggggcgggat ttcatggata tgtttctttc tgcgagaacc agccatattt aaactcttct
      361 ctcaaattta tgaatctatt atacagaaaa attttcctga aagcaaataa attttttatg
      421 atttccctcg acaattcgcg ctaacttaca ttaattgcgt tgcgcgctag cgacaccatc
      481 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
      541 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctctacg
      601 gcgaccg
//
