LOCUS       sequence-108257-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFA6a Osm1(M1A)-yoEGFP::LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Williams CC, Jan CH, Weissman JS
  TITLE     Targeting and plasticity of mitochondrial proteins revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522.
  PUBMED    25378625
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-11-7- 7"; volume: "346"; issue: 
            "6210"; pages: "748-51"
COMMENT     Sequence Label: pJW1516
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(55..765,766..768))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc cttatttgta
       61 caattcatcc atacccaacg ttatacccgc agcggtgaca aattctaaca aaaccatgtg
      121 atctctcttt tcattaggat cttttgacaa ggcgctctgt gtagataagt aatggttgtc
      181 tggcaataga acaggtccat ctccgattgg tgtattttgt tgataatgat cagcgagttg
      241 aacggaacca tcttcgatat tatgtctaat tttgaaatta acttttatgc cgtttttctg
      301 tttatctgcc atgatgtaaa cgttatgtga attgtagttg tattccaatt tgtgacctaa
      361 gatattacca tcttccttaa agtcaatacc ctttaactca attctattaa caagtgtatc
      421 tccttcaaac ttcacttcag cacgagtttt gtaattacca tcatctttaa agaaaatagt
      481 tctctcttgc acatatccct caggcatggc agatttgaaa aagtcatgtt gtttcatgtg
      541 gtctgggtat cgagaaaaac attgtactcc gtaagtgaga gttgtaacta gagttggcca
      601 tgggacaggc agcttccctg tggtgcagat gaacttaagt gtaagtttgc cataagttgc
      661 gtccccttca ccctcaccac tcacagaaaa cttatgcccg tttacgtcgc catccaattc
      721 taccagtatt ggtacgacac ctgtgaatag ttcctcgcct tttgacatgt taattaaacc
      781 agcaccgtca ccgtacaatt ttgctatgtt atccgcagct gtctttccaa agacaacaca
      841 ctctaacaaa ctagatccac ccaatctgtt ggctccatga acaccacccg aaacttcacc
      901 agcagcaaat atcccattag atagaacgct t
//
