LOCUS       sequence-108263-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent bile acid sensor (BAS) targeted to nucleus that allows 
            spatiotemporal monitoring of bile acid transport in single living 
            cells using FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   van der Velden LM, Golynskiy MV, Bijsmans IT, van Mil SW, Klomp LW, 
            Merkx M, van de Graaf SF
  TITLE     Monitoring bile acid transport in single living cells using a 
            genetically encoded Forster resonance energy transfer sensor.
  JOURNAL   Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013
            Jan 8.
  PUBMED    22899095
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/hep.26012";
            journalName: "Hepatology"; date: "2013-02"; volume: "57"; issue: 
            "2"; pages: "740-52"
COMMENT     Sequence Label: pCMV NucleoBAS N354K-I372V
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(14..16,17..19,20..532)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(59..80)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(320..339)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagatccgct agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
       61 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggta agctgcccgt
      181 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga
      481 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
      541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      601 gcccgacaac cactacctga gcaccttctc cgccctgagc aaagacccca acgagaagcg
      661 cgatcacatg gtcctgctgg agttcctgac cgccgccggg atcactagtg aactca
//
