LOCUS       sequence-10827-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-MAPK1
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1045..1080
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 accatggcgg cggcggcggc gggcgcgggc ccggagatgg tccgcgggca ggtgttcgac
       61 gtggggccgc gctacaccaa cctctcgtac atcggcgagg gcgcctacgg catggtgtgc
      121 tctgcttatg ataatgtcaa caaagttcga gtagctatca agaaaatcag cccctttgag
      181 caccagacct actgccagag aaccctgagg gagataaaaa tcttactgcg cttcagacat
      241 gagaacatca ttggaatcaa tgacattatt cgagcaccaa ccatcgagca aatgaaagat
      301 gtatatatag tacaggacct catggaaaca gatctttaca agctcttgaa gacacaacac
      361 ctcagcaatg accatatctg ctattttctc taccagatcc tcagagggtt aaaatatatc
      421 cattcagcta acgttctgca ccgtgacctc aagccttcca acctgctgct caacaccacc
      481 tgtgatctca agatctgtga ctttggcctg gcccgtgttg cagatccaga ccatgatcac
      541 acagggttcc tgacagaata tgtggccaca cgttggtaca gggctccaga aattatgttg
      601 aattccaagg gctacaccaa gtccattgat atttggtctg taggctgcat tctggcagaa
      661 atgctttcta acaggcccat ctttccaggg aagcattatc ttgaccagct gaaccacatt
      721 ttgggtattc ttggatcccc atcacaagaa gacctgaatt gtataataaa tttaaaagct
      781 aggaactatt tgctttctct tccacacaaa aataaggtgc catggaacag gctgttccca
      841 aatgctgact ccaaagctct ggacttattg gacaaaatgt tgacattcaa cccacacaag
      901 aggattgaag tagaacaggc tctggcccac ccatatctgg agcagtatta cgacccgagt
      961 gacgagccca tcgccgaagc accattcaag ttcgacatgg aattggatga cttgcctaag
     1021 gaaaagctca aagaactaat ttttgaagag actgctagat tccagccagg atacagatct
     1081 ttgc
//
