LOCUS       sequence-108276-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging 
            plasmid for making Integrase deficient lentiviral vectors since has 
            a point mutation in the integrase gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: psPAX2-D64V
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(259..285)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(579..623)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggttgtttc gagcttatag caaaatcctt tccaagccct gtcttattct tctaggtatg
       61 tggcgaatag ctctacaagc tccttgtact acttctataa ccctatctgt cccctcagct
      121 actgctatgg ctgtggcatt gagcaagcta acagcactat tctttagctc ctgactccaa
      181 tattgtagga gattccacca atatttgagg gcttcccacc ccctgcgtcc cagaagttcc
      241 acaatcctcg ttacaatcaa gagtaagtct ctcaagcggt ggtagctgaa gaggcacagg
      301 ctccgcagat cgtcccagat aagtgccaag gatccgttca ctaatcgaat ggatctgtct
      361 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
      421 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
      481 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
      541 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
      601 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
      661 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
      721 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
      781 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
      841 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
      901 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
      961 ctggcctgta
//
