LOCUS       sequence-108279-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging 
            plasmid for making Integrase deficient lentiviral vectors since has 
            a point mutation in the integrase gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: psPAX2-D64V
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    815..1048
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
      121 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
      181 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
      241 acacatggaa ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc
      301 agaataggcg ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga
      361 gccctggaag catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa
      421 gtgttgcttt cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag
      481 gaagaagcgg agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct
      541 atcaaagcag taagtagtac atgtaatgca acctataata gtagcaatag tagcattagt
      601 agtagcaata ataatagcaa tagttgtgtg gtccatagta atcatagaat ataggaaaat
      661 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
      721 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
      781 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
      841 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
      901 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
      961 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1021 aaagatacct aaaggatcaa cagctcctgg gg
//
