LOCUS       sequence-108284-         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express codon optimized Lin-28 and wild type Nanog. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    363..587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
       61 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      121 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      181 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      241 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      301 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aagctttaat
      361 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      421 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg cgtcgcattg
      481 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      541 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggaat
//
