LOCUS       sequence-108285-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express codon optimized Lin-28 and wild type Nanog. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcagcaccta cctaccccag cctctactct tcctaccacc agggatgcct ggtgaacccg
       61 actgggaacc ttccaatgtg gagcaaccag acctggaaca attcaacctg gagcaaccag
      121 acccagaaca tccagtcctg gagcaaccac tcctggaaca ctcagacctg gtgcacccaa
      181 tcctggaaca atcaggcctg gaacagtccc ttctataact gtggagagga atctctgcag
      241 tcctgcatgc agttccagcc aaattctcct gccagtgact tggaggctgc tttggaagct
      301 gctggggaag gccttaatgt aatacagcag accactaggt attttagtac tccacaaacc
      361 atggatttat tcctaaacta ctccatgaac atgcaacctg aagacgtgtg aaactagtgg
      421 atctccacgt ggcggctagt actccggtat tgcggtaccc ttgtacgcct gttttatact
      481 cccttcccgt aacttagacg cacaaaacca agttcaatag aagggggtac aaaccagtac
      541 caccacgaac aagcacttct gtttccccgg tgatgtcgta tagactgctt gcgtggttga
      601 aagcgacgga tccgttatcc gcttatgtac ttcgagaagc ccagtaccac ctcggaatct
      661 tcgatgcgtt gcgctcagca ctcaacccca gagtgtagct taggctgatg agtctggaca
      721 tccctcaccg gtgacggtgg tccaggctgc gttggcggcc tacctatggc t
//
