LOCUS       sequence-108286-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    24..76
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(616..715)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(645..665)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
       61 gaagcttgag ctcgagagtg ctcccgccac tgccctccga gggcaccgcg gccggactgg
      121 caggcgccgc cggggtgcgg ggggcagcgt ttcgcgcgcg ggcgcgggcc acgccgctgc
      181 aggacaccac gccgtggcgc cggcggaagc ggtccagcca gccgttggag gcggtgaagt
      241 cgtccatgcc cagctcctcg gctatgcgca gcgccttctc cttgaggatg atgcccttga
      301 ccggcaggcc ggcggcgcgg atctgctgga accaggcgat gagcaagccc tcgagcttgt
      361 cgtagggaga cagcttgttg gtcttgcggc aggtggaggc caccccgtac ttgcgctccg
      421 acgccaggat ggcgcgcttg ttcttcagga tcgtgctcag cgtggacggc gggatgttga
      481 agcgccgcgc gatctcgccc ttgcgtaggt ccgggttctc ctccacctcc tggatgatcc
      541 gcgacttctc ccggaacgtc agctgccgcc gcttggggcc catggtggct agcggatctg
      601 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      661 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      721 aaacaaactc ccat
//
