LOCUS       sequence-108288-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid 
            reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 227 CoMiP 4in1 wo shRNA p53
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..45)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    163..387
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
       61 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
      121 agtcggatct ccctttgggc cgcctccccg cctggactcg agctgtgcct tctagttgcc
      181 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      241 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      301 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      361 atgctgggga tgcggtgggc tctatggaga tctgatatct ctagtgaatt cagatctgag
      421 ctagctgcag taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag
      481 ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg
      541 tgagcagttt cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg
      601 gcccgaggcc aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga
      661 cccatcagat gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat
      721 taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc tgc
//
