LOCUS       sequence-108289-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid 
            reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 227 CoMiP 4in1 wo shRNA p53
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        179..585
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
       61 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
      121 gcggtgggct ctatggagat ctgatatctc tagtgaattc agatctgagc tagctgcagt
      181 aacgccattt tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag
      241 ggcgggtaca tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc
      301 ggccccggcc cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca
      361 agaacagatg gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg
      421 tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag
      481 cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa
      541 cccctcactc ggcgcgccag tcctccgaca gactgagtcg gccggtggat ccaccggtat
      601 tgggatcttc acacagcagg taaggttgcg ggccgggcct gggccgggtc cgggccgggt
      661 attgcccgcc taatgagc
//
