LOCUS       sequence-108292-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(94..500)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gctcattagg cgggcaatac ccggcccgga cccggcccag gcccggcccg caaccttacc
       61 tgctgtgtga agatcccaat accggtggat ccaccggccg actcagtctg tcggaggact
      121 ggcgcgccga gtgaggggtt gtgagctctt ttatagagct cgggaagcag aagcgcgcga
      181 acagaagcga gaagcaggct gattggttaa ttcaaataag gcgcagggtc atttcaggtc
      241 cttgggggag cctggaaaca tctgatgggt cttaagaaac tgctgagggt tgggccatat
      301 ctggggacca tctgttcttg gcctcgggcc ggggccgaaa ctgcggtgac catctgttct
      361 tggccccggg ccggggccga aactgctcac cgcagatatc ctgtttggcc caacgttagc
      421 tattttcatg tacccgccct tgatctgaac ttctctattc ttggtttggt atttttccat
      481 gccttgcaaa atggcgttac tgcagctagc tcagatctga attcactagt cgcgcccggg
      541 gagcccaaag gttaccccag ttggggcggg cccccatggg tcgactctag tgacgccgcc
      601 atctctaggc ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc
      661 tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa
      721 aagacagata atctgttctt tttaatacta gctacatttt acatgatagg c
//
