LOCUS       sequence-108293-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..60)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    178..402
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(560..580)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
       61 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
      121 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg actcgagctg
      181 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      241 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      301 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      361 aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatctga tatctctagt
      421 cccgggctgc aggaattcca aaaaagactc cagtggtaat ctactctctt gaagtagatt
      481 accactggag tcggcccaaa caaggctttt ctccaaggga tatttatagt ctcaaaacac
      541 acaattactt tacagttagg gtgagtttcc ttttgtgctg ttttttaaaa taataattta
      601 gtatttgtat ctcttataga aatccaagcc tatcatgtaa aatgtagcta gtattaaaaa
      661 gaacagatta tctgtctttt atcgcacatt aagcctctat agttacta
//
