LOCUS       sequence-108294-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP 
            from EFS promoter. 3rd generation lentiviral backbone. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song 
            J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA
  TITLE     Genome-wide CRISPR screen in a mouse model of tumor growth and 
            metastasis.
  JOURNAL   Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. 
            Epub 2015 Mar 5.
  PUBMED    25748654
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.02.038"; journalName: "Cell"; date: 
            "2015-03-12- 12"; volume: "160"; issue: "6"; pages: "1246-60"
COMMENT     Sequence Label: lentiCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat
       61 cagcttggcg ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga
      121 gggcacgttg tcgctcttgc cccggttctt gtcgcttctg gtcagcacct tgttgtcgat
      181 ggagtcgtcc ttcagaaagc tctgaggcac gatatggtcc acatcgtagt cggacagccg
      241 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
      301 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
      361 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
      421 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
      481 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
      541 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
      601 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
      661 tctgttggcg aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc
      721 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
      781 cagctgcttc atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat
      841 catctctctg tcctcaaaca gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt
      901 ttcctcattg tccaggaagt ccttgtcctt gataattttc agcagatcgt ggtatgtgcc
      961 cagggaggcg ttgaaccgat ctt
//
