LOCUS       sequence-108298-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP 
            from EFS promoter. 3rd generation lentiviral backbone. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song 
            J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA
  TITLE     Genome-wide CRISPR screen in a mouse model of tumor growth and 
            metastasis.
  JOURNAL   Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. 
            Epub 2015 Mar 5.
  PUBMED    25748654
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.02.038"; journalName: "Cell"; date: 
            "2015-03-12- 12"; volume: "160"; issue: "6"; pages: "1246-60"
COMMENT     Sequence Label: lentiCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(333..544)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    637..754
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct gaagatctct
       61 tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt tctcttcagc
      121 cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag gttcttcttg
      181 atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac cttgtactcg
      241 tcggtgatca cggcccagcc cacagagttg gtgccgatgt ccaggccgat gctgtacttc
      301 ttgtccatgg tggcagcgct ctagaaccgg tcctgtgttc tggcggcaaa cccgttgcga
      361 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
      421 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
      481 ccggatcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
      541 gcccactgac gggcaccgga gccaattccc actcctttca agacctagct agctaattaa
      601 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
      661 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
      721 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
      781 gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc
      841 tctctccacc ttcttcttct attccttcgg gcctg
//
