LOCUS       sequence-108299-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP 
            from EFS promoter. 3rd generation lentiviral backbone. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song 
            J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA
  TITLE     Genome-wide CRISPR screen in a mouse model of tumor growth and 
            metastasis.
  JOURNAL   Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. 
            Epub 2015 Mar 5.
  PUBMED    25748654
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.02.038"; journalName: "Cell"; date: 
            "2015-03-12- 12"; volume: "160"; issue: "6"; pages: "1246-60"
COMMENT     Sequence Label: lentiCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             30..210
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    257..382
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
       61 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      121 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      181 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      241 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      301 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      361 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      421 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
      481 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
      541 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
      601 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
      661 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
      721 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
      781 ataaatataa agta
//
