LOCUS       sequence-108303-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC41
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             411..560
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tgcccaaaaa gaaaagaaaa gtgggtagta tgtccaaaac catcgttctt tcggtcggcg
       61 aggctactcg cactctgact gagatccagt ccaccgcaga ccgtcagatc ttcgaagaga
      121 aggtcgggcc tctggtgggt cggctgcgcc tcacggcttc gctccgtcaa aacggagcca
      181 agaccgcgta tcgcgtcaac ctaaaactgg atcaggcgga cgtcgttgat tccggacttc
      241 cgaaagtgcg ctacactcag gtatggtcgc acgacgtgac aatcgttgcg aatagcaccg
      301 aggcctcgcg caaatcgttg tacgatttga ccaagtccct cgtcgcgacc tcgcaggtcg
      361 aagatcttgt cgtcaacctt gtgccgctgg gccgtggctc cggacgagcc gatgctctcg
      421 acgattttga cctcgatatg ctgggatccg acgctctcga tgacttcgac cttgacatgc
      481 tggggagtga cgctctggac gacttcgatc tcgacatgct gggctccgac gccctggatg
      541 acttcgatct ggacatgctc atcaactgac ccgggtcgag catgcatcta gggcggccaa
      601 ttccgcccct ctccctcccc cccccc
//
