LOCUS       sequence-10831-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-BRAF
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaaggatttt ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagt tggcaccatg gcggcgctga gcggtggcgg
      121 tggtggcggc gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg
      181 cgccggcgcc ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg
      241 gaatatcaaa caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt
      301 tggtggggag cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa
      361 gctagatgca ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga
      421 tttttctgtt tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct
      481 ttcagtgcta ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa
      541 ccccaagtca ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt
      601 ggtacctgca aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag
      661 aggtctaatc ccagagtgct gtgctgttta cagaattcat gatgggtctt ttctggagct
      721 gacttgaaga aaagagtaca tctctttacc ctgctgtttg tccaagagtg atacatttat
      781 ttgggagaga ag
//
