LOCUS       sequence-108315-         446 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC101
FEATURES             Location/Qualifiers
     source          1..446
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             231..380
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tgcccaaaaa gaaaagaaaa gtgggtagta tgaaatcaat tcgctgtaaa aactgcaaca
       61 aactgttatt taaggcggat agttttgatc acattgaaat caggtgtccg cgttgcaaac
      121 gtcacatcat aatgctgaat gcctgcgagc atcccacgga gaaacattgt gggaaaagag
      181 aaaaaatcac gcattctgac gaaaccgtgc gttatggctc cggacgagcc gatgctctcg
      241 acgattttga cctcgatatg ctgggatccg acgctctcga tgacttcgac cttgacatgc
      301 tggggagtga cgctctggac gacttcgatc tcgacatgct gggctccgac gccctggatg
      361 acttcgatct ggacatgctc atcaactgac ccgggtcgag catgcatcta gggcggccaa
      421 ttccgcccct ctccctcccc cccccc
//
